Most molecules have a net charge within a pH range of 2 to 10. When the pH is altered, the net charge on molecules can change drastically. In this Ion Exchange Chromatography Kit, a mixture of two chemicals is absorbed onto a solid support ion-exchange column and separated during elution under conditions that influence their net charge. Group Size: for 10 lab groups. Storage: Room temperature. Time Required: Complete in 60-90 minutes. The kit includes:. Full instructions. Ion exchanger. Chemical mixture. Potassium Acetate buffer. Chromatography columns. Additional required equipment & materials (not supplied with the kit):. Spectrophotometer (B8L62443). Cuvettes (B8A60069 - 100pack). Retort Stand with clamp (B8R06571). Test tubes (B8R07897 - 100pack). Graduated glass pipette: (B8R05901). Pipette filler (B8A78062).